为了揭示银杏种子生长发育对矿物质养分的需求规律,研究了不同生长发育时期银杏种子中N、P、K、Ca、Mg等矿质营养元素含量的季节变化。结果表明,银杏种子中N、P、K、Ca、Mg元素的含量随时间呈从高到低的规律性变化,但不同元素间变化的幅度不同,整个生长季中4月底至5月下旬和5月下旬至6月上旬银杏种子中营养元素含量变化较大。微量元素的含量变化各有其特点。同一时期正常发育种子和脱落种子中营养元素含量存在明显差异。成熟种子中8种主要营养元素含量为N10.4g/kg,P1.3g/kg,K14.9g/kg,Ca1.3g/kg,Mg0.9g/kg,Mn3.2mg/kg,Zn27.6mg/kg,Fe10.0mg/kg.

The relationship among growing process,drops and endogenous hormones contents on Ginkgo biloba seeds were studied. The results showed that the growing curve of seed is S shape. The period of seed expanding late from the beginning of May to the middle of July. There is a remarkable relation between seed growth and content of endogenous hormones in seeds. The contents of IAA,ZR and ABA are higher whereas GA 3 is lower in the period of flourishing florescence, then the content of IAA,ZR and ABA significantly decreased but GA 3 increases after pollination. In the period of seed expanding, the content of IAA and ZT gradually increases again, and reaches another peak at the beginning of July, then both of them steady decrease, but at the same time the content of ABA gradually increases until the seed ripe. In a word, the function of GA 3,IAA,ZR and ZT seem to prompt the seed growth, whereas ABA concerns with seed mature and drops. There are two times drops of Ginkgo biloba seeds. The drops are related to hormones.

研究了印楝细胞悬浮培养系的建立及印楝细胞悬浮培养的条件。结果表明,印楝细胞悬浮系的建立以B5培养基,NAA2.0～4.0mg·L-1,采用直接倾倒法作为接种方法为好;基于生物量、印楝素含量和印楝素产率综合分析认为,印楝细胞悬浮培养的最佳条件为B5培养基,NAA2～4mg·L-1,蔗糖30g·L-1,培养温度25℃,及根据培养容器的大小选择合适的转速和装液量。